Biology of Blood and Marrow Transplantation
Volume 9, Issue 9 , Pages 559-570 , September 2003

An epithelial target site in experimental graft-versus-host disease and cytokine-mediated cytotoxicity is defined by cytokeratin 15 expression

  • Diana Whitaker-Menezes

      Affiliations

    • Departments of Pathology, Anatomy & Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
    • ,
  • Stephen C Jones

      Affiliations

    • Department of Microbiology & Immunology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
    • ,
  • Thea M Friedman

      Affiliations

    • Department of Microbiology & Immunology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
    • ,
  • Robert Korngold

      Affiliations

    • Department of Microbiology & Immunology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
    • ,
  • George F Murphy

      Affiliations

    • Departments of Pathology, Anatomy & Cell Biology, Thomas Jefferson University, Philadelphia, Pennsylvania, USA
    • Corresponding Author InformationCorrespondence and reprint requests: George F. Murphy, MD, Department of Pathology, Anatomy & Cell Biology, Thomas Jefferson University, 1020 Locust St., Rm. 545 JAH, Philadelphia, PA 19107–6799, USA

Received 2 July 2003 ,Accepted 1 August 2003.

References 

  1. Gilliam AC, Murphy GF. Cellular pathology of cutaneous graft-versus-host disease. In:  Ferrara JLM,  Deeg HI,  Burakoff SJ editor. Graft-vs-Host Disease.: Immunology, Pathophysiology, and Treatment. New York, NY: Marcel Dekker; 1996;p. 291–313
  2. Gilliam A, Whitaker-Menezes D, Korngold R, Murphy GF. Apoptosis is the predominant form of epithelial target cell injury in acute experimental graft-versus-host disease. J Invest Dermatol. 1996;107:377–383
  3. Yoo YH, Gilliam A, Whitaker-Menezes D, Korngold R, Murphy GF. Experimental induction and ultrastructural characterization of apoptosis in murine acute graft-versus-host disease. Arch Dermatol Res. 1997;289:389–398
  4. Sale GE, Shulman HM, Gallucci BB, Thomas ED. Young rete ridge keratinocytes are preferred targets in cutaneous graft-vs-host disease. Am J Pathol. 1985;118:278–287
  5. Sale GE, Raff RF, Storb R. Stem cell regions in filiform papillae of tongue as targets of graft-vs-host disease. Transplantation. 1994;58:1273–1287
  6. Kim JC, Whitaker-Menezes D, Deguchi M, Adair B, Korngold R, Murphy GF. Novel expression of VCAM-1 (CD106) by squamous epithelium in experimental acute graft-versus-host disease. Am J Pathol. 2002;161:763–769
  7. Sale GE, Beauchamp MD, Akiyama M. Parafollicular bulges, but not hair bulb keratinocytes, are attacked in graft-vs-host disease of human skin. Bone Marrow Transplant. 1994;14:411–413
  8. Murphy GF, Lavker RM, Whitaker D, Korngold R. Cytotoxic folliculitis in GVHD. J Cutan Pathol. 1990;18:309–314
  9. Lavker RM, Sun T-T. Heterogeneity in epidermal basal keratinocytes (morphological and functional correlations). Science. 1982;215:1239–1241
  10. Cotsarelis G, Sun T-T, Lavker RM. Label-retaining cells reside in the bulge area of pilo-sebaceous unit (implications for follicular stem cells, hair cycle, and skin carcinogenesis). Cell. 1990;61:1329–1337
  11. Bickenback JR. Identification and behavior of label-retaining cells in oral mucosa and skin. J Dent Res. 1981;60:1611–1620
  12. Korngold R, Sprent J. Lethal graft-vs-host disease following bone marrow transplantation across minor histocompatibility barriers in mice. Prevention by removing mature T cells from marrow. J Exp Med. 1978;148:1687–1698
  13. Ferrara J, Guillen FJ, Sleckman B, Burakoff SJ, Murphy GF. Cutaneous acute graft-vs-host disease to minor histocompatibility antigens in a murine model (histologic analysis and correlation to clinical disease). J Invest Dermatol. 1986;86:371–375
  14. Murphy GF, Whitaker D, Sprent J, Korngold R. Characterization of target injury of murine acute graft-vs-host disease directed to multiple minor histocompatibility antigens elicited by either CD4+ or CD8+ effector cells. Am J Pathol. 1991;138:983–990
  15. Murphy GF, Sueki H, Teuscher C, Whitaker D, Korngold R. Role of mast cells in early epithelial target cell injury in experimental acute graft-versus-host disease. J Invest Dermatol. 1994;102:451–461
  16. Lyle S, Christofidou-Solomidou M, Liu Y, Elder D, Albelda S, Cotsarelis G. The C8/144B monoclonal antibody recognizes cytokeratin 15 and defines the location of human hair follicle stem cells. J Cell Sci. 1998;111:3179–3188
  17. Waseem A, Dogan B, Tidman N, et al.  Keratin 15 expression in stratified epithelia (downregulation in activated keratinocytes). J Invest Dermatol. 1999;112:362–369
  18. Whitbread IA, Powell BC. Expression of the intermediate filament keratin gene, K15, in the basal cell layers of epithelia and the hair follicle. Exp Cell Res. 1998;244:448–459
  19. Friedman TM, Statton D, Jones SC, Berger MA, Murphy GF, Korngold R. Vβ spectratype analysis reveals heterogeneity of CD4+ T cell responses to minor histocompatibility antigens involved in graft-versus-host disease (correlations with epithelial tissue infiltrate). Biol Blood Marrow Transplant. 2001;7:2–13
  20. Friedman TM, Gilbert M, Briggs C, Korngold R. Repertoire analysis of CD8+ T cell responses to minor histocompatibility antigens involved in graft-versus-host disease. J Immunol. 1998;161:41–48
  21. Casanova JL, Romero P, Widmann C, Kourilsky P, Maryanski JL. T cell receptor genes in a series of class I major histocompatibility complex-restricted cytotoxic T lymphocyte clones specific for a Plasmodium berghei nonapeptide (implications for T cell allelic exclusion and antigen-specific repertoire). J Exp Med. 1991;174:1371–1383
  22. Karnovsky MJ. A formaldehyde-glutaraldehyde fixative of high osmolarity for use in electron microscopy. J Cell Biol. 1965;27:137A; (abstr.)
  23. Mbiene J-P, MacCallum DK, Mistretta CM. Organ cultures of embryonic rat tongue support tongue and gustatory papilla morphogenesis in vitro without intact sensory ganglia. J Comp Neurol. 1997;377:324–340
  24. Stoitzner P, Zanella M, Ortner U, et al.  Migration of Langerhans cells and dermal dendritic cells in skin organ cultures (augmentation by TNFα and IL-1β). J Leukoc Biol. 1999;66:462–470
  25. Messadi DV, Pober JD, Fiers W, Gimbrone MA, Murphy GF. Induction of an activation antigen on postcapillary venular endothelium in human skin organ culture. J Immunol. 1987;139:1557–1562
  26. Korngold R, Jameson B, McDonnell JM, et al.  Peptide analogs which inhibit IgE-FcϵRIα interactions ameliorate the development of lethal graft-versus-host disease. Biol Blood Marrow Transplant. 1997;3:187–193
  27. Van den Brink MRM, Moore E, Horndasch KJ, Crawford JM, Murphy GF, Burakoff SJ. Fas ligand-deficient gld mice are more susceptible to graft-versus-host disease. Transplantation. 2000;70:184–191
  28. Van den Brink MRM, Moore E, Crawford JM, Hoffman J, Murphy GF, Burakoff SJ. Fas-deficient lpr mice are more susceptible to graft-versus-host disease. J Immunol. 2000;164:469–480
  29. Schmaltz C, Alpdogan O, Kappel BJ, et al.  T cells require TRAIL for optimal graft-versus-tumor activity. Nat Med. 2002;8:1433–1437
  30. Teshima T, Ordemann R, Reddy P, et al.  Acute graft-versus-host disease does not require alloantigen expression on host epithelium. Nat Med. 2002;8:575–581
  31. Klein LM, Lavker RM, Matis WL, Murphy GF. Degranulation of human mast cells induces an endothelial antigen central to leukocyte adhesion. Proc Natl Acad Sci U S A. 1989;86:8972–8976
  32. Walsh LJ, Trinchieri G, Waldorf HA, Whitaker D, Murphy GF. Human dermal mast cells contain and release tumor necrosis factor-α which induces endothelial leukocyte adhesion molecule-1. Proc Natl Acad Sci U S A. 1991;88:4220–4224
  33. Korngold R, Sprent J. Features of T cells causing H-2 restricted lethal graft-versus-host disease across minor histocompatibility barriers. J Exp Med. 1982;155:872–883
  34. Elices MJ, Osborn L, Takada Y, et al.  VCAM-1 on activated endothelium interacts with the leukocyte integrin VLA-4 at a site distinct from the VLA/fibronectin binding site. Cell. 1990;60:577–584
  35. Honda S, Campbell JJ, Andrew DP, et al.  Ligand-induced adhesion to activated endothelium and to vascular cell adhesion molecule-1 in lymphocytes transfected with the N-formyl peptide receptor. J Immunol. 1994;152:4026–4035
  36. Damle NK, Klussman K, Linsley PS, Aruffo A. Differential costimulatory effects of adhesion molecules B7, ICAM-1, LFA-3, and VCAM-1 on resting and antigen-primed CD4+ T lymphocytes. J Immunol. 1992;148:1985–1992
  37. Sale GE. Are stem cells attacked in graft-versus-host-disease?. J Cutan Pathol. 1991;18:305–306
  38. Lavker RM, Miller SJ, Sun T-T. Epithelial stem cells, hair follicles and tumor formation. Recent Results Cancer Res. 1993;128:31–43
  39. Cotsarelis G, Cheng S-Z, Dong G, Sun T-T, Lavker RM. Existence of slow-cycling limbal epithelial basal cells that can be preferentially stimulated to proliferate (implications on epithelial stem cells). Cell. 1998;57:201–209
  40. Werner S, Werner S, Munz B. Suppression of keratin 15 expression by transforming growth factor β in vitro and by cutaneous injury in vivo. Exp Cell Res. 2000;254:80–90
  41. Jakic-Razumovic J, Browne MD, Sale GE. Proliferation rates in epidermis of patients with graft-versus-host disease, non-specific inflammation and normal skin. Bone Marrow Transplant. 1992;10:27–31
  42. Piguet PF, Grau GE, Allet B, Vassalli P. Tumor necrosis factor/cachectin is an effector of skin and gut lesions of the acute phase of graft-vs-host disease. J Exp Med. 1987;166:1280–1289
  43. Korngold R, Marini J, de Baca ME, Murphy GF, Giles-Komar J. Role of tumor necrosis factor-α and graft-versus-host disease and graft-versus-leukemia responses. Biol Blood Marrow Transplant. 2003;9:292–303
  44. Tartaglia LA, Goeddel DV. Two TNF receptors. Immunol Today. 1992;13:151–153
  45. Ruckert R, Linder G, Bulfone-Paus S, Paus R. High-dose proinflammatory cytokines induce apoptosis of hair bulb keratinocytes in vivo. Br J Dermatol. 2000;143:1036–1039

PII: S1083-8791(03)00288-X

doi: 10.1016/S1083-8791(03)00288-X

Biology of Blood and Marrow Transplantation
Volume 9, Issue 9 , Pages 559-570 , September 2003

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