Therapeutic and Diagnostic Applications of Minor Histocompatibility Antigen HA-1 and HA-2 Disparities in Allogeneic Hematopoietic Stem Cell Transplantation: A Survey of Different Populations

Monitoring of disease recurrence by tracing the patient-specific HA-2^M allele in patient 20. Genomic HA-2 typing of patient 20 and her haploidentical family stem cell donor was performed as described in “Materials and Methods.” PCR fragments of 327 and 274 bp correspond to the internal control gene and the HA-2 allele, respectively. M and V stand for the polymorphism HA-2^M and HA-2^V, respectively. Agarose gel electrophoresis is shown of PCR products obtained from peripheral blood of the donor (lane 1), of the patient before transplantation (lane 2), and of bone marrow aspirates from the patient drawn in complete clinical remission at day +90 (lane 3) or in relapse at day +150 (lane 4) after HSCT. —, PCR mix amplified in the absence of template DNA. The molecular weight marker used is GeneRuler 50bp DNA ladder (MBI Fermentas, Hanover, MD).