Biology of Blood and Marrow Transplantation
Volume 12, Issue 3 , Pages 267-274 , March 2006

Isolation of CD4+CD25+ Regulatory T Cells for Clinical Trials

  • Petra Hoffmann

      Affiliations

    • Department of Hematology and Oncology, University Hospital Regensburg, Regensburg, Germany
    • Institute of Immunology, University Regensburg, Regensburg, Germany
    • ,
  • Tina J. Boeld

      Affiliations

    • Department of Hematology and Oncology, University Hospital Regensburg, Regensburg, Germany
    • ,
  • Ruediger Eder

      Affiliations

    • Department of Hematology and Oncology, University Hospital Regensburg, Regensburg, Germany
    • ,
  • Julia Albrecht

      Affiliations

    • Department of Hematology and Oncology, University Hospital Regensburg, Regensburg, Germany
    • ,
  • Kristina Doser

      Affiliations

    • Department of Hematology and Oncology, University Hospital Regensburg, Regensburg, Germany
    • ,
  • Biserka Piseshka

      Affiliations

    • Department of Hematology and Oncology, University Hospital Regensburg, Regensburg, Germany
    • ,
  • Ashraf Dada

      Affiliations

    • Institute for Clinical Chemistry and Laboratory Medicine, University Hospital Regensburg, Regensburg, Germany
    • ,
  • Claudia Niemand

      Affiliations

    • Miltenyi Biotec, Bergisch Gladbach, Germany
    • ,
  • Mario Assenmacher

      Affiliations

    • Miltenyi Biotec, Bergisch Gladbach, Germany
    • ,
  • Evelyn Orsó

      Affiliations

    • Institute for Clinical Chemistry and Laboratory Medicine, University Hospital Regensburg, Regensburg, Germany
    • ,
  • Reinhard Andreesen

      Affiliations

    • Department of Hematology and Oncology, University Hospital Regensburg, Regensburg, Germany
    • ,
  • Ernst Holler

      Affiliations

    • Department of Hematology and Oncology, University Hospital Regensburg, Regensburg, Germany
    • ,
  • Matthias Edinger

      Affiliations

    • Department of Hematology and Oncology, University Hospital Regensburg, Regensburg, Germany
    • Corresponding Author InformationCorrespondence and reprint requests: Matthias Edinger, MD, Department of Hematology and Oncology, University Hospital Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany

Received 7 December 2005 ,Accepted 9 January 2006.

References 

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  2. Taylor PA , Lees CJ , Blazar BR . The infusion of ex vivo activated and expanded CD4(+)CD25(+) immune regulatory cells inhibits graft-versus-host disease lethality . Blood . 2002;99:3493–3499
  3. Cohen JL , Trenado A , Vasey D , Klatzmann D , Salomon BL . CD4(+)CD25(+) immunoregulatory T Cells (new therapeutics for graft-versus-host disease) . J Exp Med . 2002;196:401–406
  4. Edinger M , Hoffmann P , Ermann J , et al.   CD4(+)CD25(+) regulatory T cells preserve graft-versus-tumor activity while inhibiting graft-versus-host disease after bone marrow transplantation . Nat Med . 2003;9:1144–1150
  5. Ermann J , Hoffmann P , Edinger M , Dutt S , Blankenberg FG , Higgins JP , et al.   Only the CD62L+ subpopulation of CD4+CD25+ regulatory T cells protects from lethal acute GVHD . Blood . 2005;105:2220–2226
  6. Jones SC , Murphy GF , Korngold R . Post-hematopoietic cell transplantation control of graft-versus-host disease by donor CD4(+)25(+) T cells to allow an effective graft-versus-leukemia response . Biol Blood Marrow Transplant . 2003;9:243–256
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  9. Jonuleit H , Schmitt E , Stassen M , Tuettenberg A , Knop J , Enk AH . Identification and functional characterization of human CD4(+)CD25(+) T cells with regulatory properties isolated from peripheral blood . J Exp Med . 2001;193:1285–1294
  10. Taams LS , Smith J , Rustin MH , Salmon M , Poulter LW , Akbar AN . Human anergic/suppressive CD4(+)CD25(+) T cells (a highly differentiated and apoptosis-prone population) . Eur J Immunol . 2001;31:1122–1131
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  15. Walker MR , Kasprowicz DJ , Gersuk VH , et al.   Induction of FoxP3 and acquisition of T regulatory activity by stimulated human CD4+CD25− T cells . J Clin Invest . 2003;112:1437–1443
  16. Fontenot JD , Gavin MA , Rudensky AY . Foxp3 programs the development and function of CD4+CD25+ regulatory T cells . Nat Immunol . 2003;4:330–336
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PII: S1083-8791(06)00043-7

doi: 10.1016/j.bbmt.2006.01.005

Biology of Blood and Marrow Transplantation
Volume 12, Issue 3 , Pages 267-274 , March 2006

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