Allogeneic Stem Cell Transplantation with Peripheral Blood Stem Cells Mobilized by Pegylated G-CSF

Abstract 

Mobilization of stem cells with pegylated granulocyte colony-stimulating factor (peg-G-CSF) modulates donor T- and natural killer T-cell (NKT-cell) functions, thus separating graft-versus-host from graft-versus-leukemia disease in animal models. We report a phase I/II study that analyzed the feasibility of mobilizing stem cells from normal donors with peg-G-CSF and the ability of these cells to restore hematopoiesis in allogeneic transplant recipients after myeloablative conditioning. Administration of 6 mg of peg-G-CSF resulted in suboptimal stem cell mobilization, with a peak peripheral blood CD34⁺ count of 29 ± 5/μL. Apheresis 4 days after peg-G-CSF yielded 2.7 ± .4 × 10⁶ CD34⁺ cells/kg recipient weight, and all donors required a second collection on day 5 to yield a total of 4.2 ± .5 × 10⁶ CD34⁺ cells/kg recipient weight. After escalation of the dose to 12 mg, the peak CD34⁺ count was 99 ± 11/μL and 12 of 13 donors collected sufficient stem cells for transplantation in a single apheresis (8.9 ± 1.4 × 10⁶ CD34⁺ cells/kg recipient weight). Late transient increases in serum hepatic transaminases were noted, but other side effects (predominantly bone pain) were otherwise similar to those seen in donors mobilized with standard G-CSF. Median neutrophil and platelet engraftments occurred on days 18 and 14, respectively, after transplantation and were identical to those seen with in recipients of grafts mobilized with standard G-CSF. With a median follow-up of 357 days, the incidence of grade II-IV acute graft-versus-host disease was 50% and there have been no relapses to date. Mobilization of stem cells with peg-G-CSF in normal donors is feasible and 12 mg results in mobilization characteristics similar to those of standard G-CSF.

Key Words:  Stem cell mobilization , Pegylated G-CSF , Graft-versus-host disease