Biology of Blood and Marrow Transplantation
Volume 13, Issue 2 , Pages 145-150 , February 2007

Umbilical Cord Blood Produces Small Megakaryocytes After Transplantation

  • Mark Ignatz

      Affiliations

    • Department of Pediatrics, University of Florida, Gainesville, Florida
  • ,
  • Martha Sola-Visner

      Affiliations

    • Department of Pediatrics, University of Florida, Gainesville, Florida
  • ,
  • Lisa M. Rimsza

      Affiliations

    • Department of Pathology, University of Arizona, Tucson, Arizona
  • ,
  • Debra Fuchs

      Affiliations

    • Department of Pathology, University of Arizona, Tucson, Arizona
  • ,
  • Jonathan J. Shuster

      Affiliations

    • Department of Epidemiology and Health Policy Research and General Clinical Research Center, University of Florida, Gainesville, Florida
  • ,
  • Xiao-Miao Li

      Affiliations

    • Department of Pediatrics, University of Florida, Gainesville, Florida
  • ,
  • Anil Jotwani

      Affiliations

    • Department of Pediatrics, University of Florida, Gainesville, Florida
  • ,
  • Susan Staba

      Affiliations

    • Department of Pediatrics, University of Florida, Gainesville, Florida
  • ,
  • John R. Wingard

      Affiliations

    • Blood and Marrow Transplant Program, University of Florida, Gainesville, Florida
  • ,
  • Zhongbo Hu

      Affiliations

    • Department of Pediatrics, University of Florida, Gainesville, Florida
  • ,
  • William B. Slayton

      Affiliations

    • Department of Pediatrics, University of Florida, Gainesville, Florida
    • Shands Cancer Center Program in Stem Cell Biology and Regenerative Medicine, University of Florida, Gainesville, Florida
    • Corresponding Author InformationCorrespondence and reprint requests: William Slayton, MD, JHMHC, Box 100296, Gainesville, FL 32610

Received 27 August 2006 ,Accepted 19 October 2006.

References 

  1. Gluckman E, Rocha V, Chevret S. Results of unrelated umbilical cord blood hematopoietic stem cell transplant. Transfus Clin Biol. 2001;8:146–154
  2. Couban S, Simpson DR, Barnett MJ, et al. A randomized multicenter comparison of bone marrow and peripheral blood in recipients of matched sibling allogeneic transplants for myeloid malignancies. Blood. 2002;100:1525–1531
  3. Sanz GF, Saavedra S, Planelles D, et al. Standardized, unrelated donor cord blood transplantation in adults with hematologic malignancies. Blood. 2001;98:2332–2338
  4. Domen RE, Hoeltge GA. Allergic transfusion reactions: an evaluation of 273 consecutive reactions. Arch Pathol Lab Med. 2003;127:316–320
  5. The Trial to Reduce Alloimmunization to Platelets Study Group. Leukocyte reduction and ultraviolet B irradiation of platelets to prevent alloimmunization and refractoriness to platelet transfusions. N Engl J Med. 1997;337:1861–1869
  6. Rozman P. Platelet antigens (The role of human platelet alloantigens (HPA) in blood transfusion and transplantation). Transplant Immunol. 2002;10:165–181
  7. Wagner SJ, Lieby D. Transfusion-associated bacterial sepsis: a concise review. Immunohematology. 1998;14:33–35
  8. Laughlin MJ, Barker J, Bambach B, et al. Hematopoietic engraftment and survival in adult recipients of umbilical cord blood from unrelated donors. N Engl J Med. 2001;344:1815–1822
  9. Wagner JE, Barker JN, DeFor TE, et al. Transplantation of unrelated donor umbilical cord blood in 102 patients with malignant and nonmalignant diseases: influence of CD34 cell dose and HLA disparity on treatment-related mortality and survival. Blood. 2002;100:1611–1618
  10. Bornstein R, Garcia-Vela J, Gilsanz F, et al. Cord blood megakaryocytes do not complete maturation, as indicated by impaired establishment of endomitosis and low expression of G1/S cyclins upon thrombopoietin-induced differentiation. Br J Haematol. 2001;114:458–465
  11. Mattia G, Vulcano F, Milazzo L, et al. Different ploidy levels of megakaryocytes generated from peripheral or cord blood CD34+ cells are correlated with different levels of platelet release. Blood. 2002;99:888–897
  12. Schipper LF, Brand A, Reniers N, et al. Differential maturation of megakaryocyte progenitor cells from cord blood and mobilized peripheral blood. Exp Hematol. 2003;31:324–330
  13. Slayton WB, Wainman DA, Li XM, et al. Developmental differences in megakaryocyte maturation are determined by the microenvironment. Stem Cells. 2005;23:1400–1408
  14. de Alarcon PA, Graeve JL. Analysis of megakaryocyte ploidy in fetal bone marrow biopsies using a new adaptation of the Feulgen technique to measure DNA content and estimate megakaryocyte ploidy from biopsy specimens. Pediatr Res. 1996;39:166–170
  15. Ma DC, Sun YH, Chang KZ, et al. Developmental change of megakaryocyte maturation and DNA ploidy in human fetus. Eur J Haematol. 1996;57:121–127
  16. Sola-Visner MC, Christensen RD, Hutson AD, and Rimsza LM. Megakaryocyte size and concentration in the bone marrow of thrombocytopenic and non-thrombocytopenic neonates. Peds Research. In press.
  17. Izumi T, Kawakami M, Enzan H, et al. The size of megakaryocytes in human fetal, infantile and adult hematopoiesis. Hiroshima J Med Sci. 1983;32:257–260
  18. Harker LA. Kinetics of thrombopoiesis. J Clin Invest. 1968;47:458–465
  19. Levine RF, Olson TA, Shoff PK, et al. Mature micromegakaryocytes: an unusual developmental pattern in term infants. Br J Haematol. 1996;94:391–399
  20. Levine RF, Hazzard KC, Lamberg JD. The significance of megakaryocyte size. Blood. 1982;60:1122–1131
  21. Williams N, Levine RF. The origin, development and regulation of megakaryocytes. Br J Haematol. 1982;52:173–180
  22. Harker LA. Thrombokinetics in man. J Clin Invest. 1969;48:963–974
  23. Bath P, Algert C, Chapman N, et al. Association of mean platelet volume with risk of stroke among 3134 individuals with history of cerebrovascular disease. Stroke. 2004;35:622–626
  24. Lehman E. In: Nonparametrics: Statistical methods based on ranks. San Francisco, CA: Holden Day; 1975;p. 175–185(1975)
  25. Slayton WB, Wainman DH, Li XM, et al. Developmental differences in megakaryocyte maturation are determined by the microenvironmentMegakaryocyte quantitation. Stem Cells. 2005;9:1400–1408
  26. Avecilla ST, Hattori K, Heissig B, et al. Chemokine-mediated interaction of hematopoietic progenitors with the bone marrow vascular niche is required for thrombopoiesis. Nat Med. 2004;10:64–71
  27. Levine RF, Bunn PA, Hazzard KC, et al. Flow cytometric analysis of megakaryocyte ploidy: comparison with Feulgen microdensitometry and discovery that 8N is the predominant ploidy class in guinea pig and monkey marrow. Blood. 1980;56:210–217
  28. Brichard B, Vermylen C, Ninane J, et al. Persistence of fetal hemoglobin production after successful transplantation of cord blood stem cells in a patient with sickle cell anemia. J Pediatr. 1996;128:241–243
  29. Honig GR, Vida LN, Hoganson GE, et al. Fetal hemoglobin expression in transplant recipients of placental blood hematopoietic progenitor cells. Pediatr Res. 1995;37:432–436
  30. Lau YL, Ma ES, Ha SY, et al. Sibling HLA-matched cord blood transplant for beta-thalassemia: report of two cases, expression of fetal hemoglobin, and review of the literature. J Pediatr Hematol Oncol. 1998;20:477–481
  31. Zhou X, Ha SY, Chan GC, et al. Successful mismatched sibling cord blood transplant in Hb Bart’s disease. Bone Marrow Transplant. 2001;28:105–107
  32. Shivdasani RA, Fujiwara Y, McDevitt MA, et al. A lineage-selective knockout establishes the critical role of transcription factor GATA-1 in megakaryocyte growth and platelet development. EMBO J. 1997;16:3965–3973
  33. Vyas P, Ault K, Jackson CW, et al. Consequences of GATA-1 deficiency in megakaryocytes and platelets. Blood. 1999;93:2867–2875
  34. Chang AN, Cantor AB, Fujiwara Y, et al. GATA-factor dependence of the multitype zinc-finger protein FOG-1 for its essential role in megakaryopoiesis. Proc Natl Acad Sci U S A. 2002;99:9237–9242
  35. Nichols KE, Crispino JD, Poncz M, et al. Familial dyserythropoietic anaemia and thrombocytopenia due to an inherited mutation in GATA1. Nat Genet. 2000;24:266–270
  36. Martin DI, Zon LI, Mutter G, et al. Expression of an erythroid transcription factor in megakaryocytic and mast cell lineages. Nature. 1990;344:444–447
  37. Miyazaki R, Ogata H, Iguchi T, et al. Comparative analyses of megakaryocytes derived from cord blood and bone marrow. Br J Haematol. 2000;108:602–609
  38. Sola MC, Du Y, Hutson AD, et al. Dose-response relationship of megakaryocyte progenitors from the bone marrow of thrombocytopenic and non-thrombocytopenic neonates to recombinant thrombopoietin. Br J Haematol. 2000;110:449–453
  39. Pastos KM, Slayton WB, Rimsza LM, et al. Differential effects of recombinant thrombopoietin and bone marrow stromal conditioned media on neonatal vs adult megakaryocytes. Blood. 2006;108:3360–3362
  40. Levine RF, Eldor A, Shoff PK, et al. Circulating megakaryocytes: delivery of large numbers of intact, mature megakaryocytes to the lungs. Eur J Haematol. 1993;51:233–246

PII: S1083-8791(06)00733-6

doi: 10.1016/j.bbmt.2006.10.032

Biology of Blood and Marrow Transplantation
Volume 13, Issue 2 , Pages 145-150 , February 2007