Hematopoietic Engraftment in Recipients of Unrelated Donor Umbilical Cord Blood Is Affected by the CD34+ and CD8+ Cell Doses

Terakura, Seitaro; Azuma, Eiichi; Murata, Makoto; Kumamoto, Tadashi; Hirayama, Masahiro; Atsuta, Yoshiko; Kodera, Yoshihisa; Yazaki, Makoto; Naoe, Tomoki; Kato, Koji

doi:10.1016/j.bbmt.2007.03.006

« Previous Next »Biology of Blood and Marrow Transplantation
Volume 13, Issue 7 , Pages 822-830, July 2007

Hematopoietic Engraftment in Recipients of Unrelated Donor Umbilical Cord Blood Is Affected by the CD34⁺ and CD8⁺ Cell Doses

Seitaro Terakura
- Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Nagoya, Japan
- Tokai Cord Blood Bank, Nagoya, Japan
- The first two authors contributed equally to this study.
Eiichi Azuma
- Department of Pediatrics and Cell Transplantation, Mie University School of Medicine, Tsu, Japan
- Tokai Cord Blood Bank, Nagoya, Japan
- The first two authors contributed equally to this study.
Makoto Murata
- Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Nagoya, Japan
- Tokai Cord Blood Bank, Nagoya, Japan
- Correspondence and reprint requests: Makoto Murata, MD, PhD, Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, Aichi 466-8550, Japan.
Tadashi Kumamoto
- Department of Pediatrics and Cell Transplantation, Mie University School of Medicine, Tsu, Japan
- Tokai Cord Blood Bank, Nagoya, Japan
Masahiro Hirayama
- Department of Pediatrics and Cell Transplantation, Mie University School of Medicine, Tsu, Japan
- Tokai Cord Blood Bank, Nagoya, Japan
Yoshiko Atsuta
- Department of Hematopoietic Stem Cell Transplantation Data Management, Nagoya University School of Medicine, Nagoya, Japan
- Tokai Cord Blood Bank, Nagoya, Japan
Yoshihisa Kodera
- Bone Marrow Transplantation Center, Japanese Red Cross Nagoya First Hospital, Nagoya, Japan
- Tokai Cord Blood Bank, Nagoya, Japan
Makoto Yazaki
- Department of Pediatrics, Nagoya Higashi Municipal Hospital, Nagoya, Japan
- Tokai Cord Blood Bank, Nagoya, Japan
Tomoki Naoe
- Department of Hematology and Oncology, Nagoya University Graduate School of Medicine, Nagoya, Japan
- Tokai Cord Blood Bank, Nagoya, Japan
Koji Kato
- Department of Pediatric Hematology/Oncology, Japanese Red Cross Nagoya First Hospital, Nagoya, Japan
- Tokai Cord Blood Bank, Nagoya, Japan

Received 19 December 2006; accepted 12 March 2007. published online 01 May 2007.

Abstract

Umbilical cord blood (UCB) transplantation is limited by the low number of hematopoietic stem cells in UCB units, which results in a low engraftment rate in transplant recipients. Here, we measured the total nucleated cell count and CD34⁺, CD3⁺, CD4⁺, CD8⁺, CD14⁺, and CD16⁺/56⁺ cell doses in each UCB unit and evaluated their influence on engraftment and other outcomes in 146 recipients. Multivariate analysis showed a significant association between a higher incidence of successful engraftment and a dose of CD34⁺ and CD8⁺ cells above the median (1.4 × 10⁵ and 15.7 × 10⁵ cells/kg, respectively). Engraftment occurred 4 days earlier in patients who received UCB with more than the median dose of CD34⁺ cells than those receiving UCB at or below the median. Stratification of the group according to CD34⁺ cell dose revealed a significant influence of the CD8⁺ cell dose on the time to achieve neutrophil engraftment in patients receiving a lower CD34⁺ cell dose, whereas there was no significant influence in the patients receiving a higher CD34⁺ cell dose. These results suggest that consideration of CD34⁺ and CD8⁺ cell doses in UCB units may improve the engraftment in recipients of UCB transplantation.