T Cell Repertoire Development in XSCID Dogs Following Nonconditioned Allogeneic Bone Marrow Transplantation

Classification of TCR Vβ spectratypes. Polyclonal Gaussian distribution (A), polyclonal skewed distribution (B), and oligoclonal distribution (C).

Immunologic reconstitution in XSCID dogs following transplantation with whole bone marrow or CD34⁺ bone marrow cells. Absolute lymphocyte counts (A), proportion of peripheral T cells (B), proportion of peripheral CD45RA⁺ (naïve) T cells (C), CD4/CD8 ratio (D), proliferative response following stimulation with PHA (E), and IgG speficic antibody response following immunization with tetanus toxoid (F).

TCR Vβ spectratypes of normal dogs and XSCID dogs following bone marrow transplantation. The normal dogs are designated with an N and the bone marrow transplanted dogs designated with B. The numbers following the N represent age of the dog in years and the numbers following the B represent the age in years following transplant.

TCR Vβ spectratyping in normal dogs and transplanted XSCID dogs. Each time point represents the summary results from an individual dog. Results are expressed as percentage of total Vβ families representing a polyclonal Gaussian (PG), polyclonal skewed (PS), or oligoclonal (O) phenotype. N-2, N-4, N-7 = normal dogs at 2, 4, and 7 years of age.

TCR Vβ spectratypes of three XSCID dogs 3 years following initial evaluation. Dog R743 was evaluated at 4.5 years and 7.5 years posttransplant. Dog X58 was evaluated at 5.5 years and 8.5 years posttransplant. Dog R468 was evaluated at 7.5 years and 10.5 years posttransplant.

TCR Vβ spectratyping in 3 transplanted XSCID dogs at initial testing and 3 years later. Results are expressed as percentage of total Vβ families representing a polyclonal Gaussian (PG), polyclonal skewed (PS), or oligoclonal (O) phenotype.