Biology of Blood and Marrow Transplantation
Volume 14, Issue 6 , Pages 693-701 , June 2008

Recipient-Derived Cells after Cord Blood Transplantation: Dynamics Elucidated by Multicolor FACS, Reflecting Graft Failure and Relapse

  • Nobukazu Watanabe

      Affiliations

    • FACS Core Laboratory, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    • Corresponding Author InformationCorrespondence and reprint requests: Nobukazu Watanabe, MD, PhD, FACS Core Laboratory, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
    • The first two authors contributed equally to this work.
  • ,
  • Satoshi Takahashi

      Affiliations

    • Research Hospital, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
    • The first two authors contributed equally to this work.
  • ,
  • Masayuki Ishige

      Affiliations

    • FACS Core Laboratory, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
  • ,
  • Yumiko Ishii

      Affiliations

    • FACS Core Laboratory, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
  • ,
  • Jun Ooi

      Affiliations

    • Research Hospital, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
  • ,
  • Akira Tomonari

      Affiliations

    • Research Hospital, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
  • ,
  • Nobuhiro Tsukada

      Affiliations

    • Research Hospital, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
  • ,
  • Takaaki Konuma

      Affiliations

    • Research Hospital, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
  • ,
  • Seiko Kato

      Affiliations

    • Research Hospital, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
  • ,
  • Aki Sato

      Affiliations

    • Research Hospital, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
  • ,
  • Arinobu Tojo

      Affiliations

    • Research Hospital, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan
  • ,
  • Hiromitsu Nakauchi

      Affiliations

    • Center for Stem Cell and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan

Received 20 February 2008 ,Accepted 7 April 2008.

References 

  1. Thiede C, Florek M, Bornhauser M, et al. Rapid quantification of mixed chimerism using multiplex amplification of short tandem repeat markers and fluorescence detection. Bone Marrow Transplant. 1999;23:1055–1060
  2. Turkiewicz D, Gorczynska E, Toporski J, et al. Monitoring of hematopoietic chimerism after sex-mismatched allogeneic stem cell transplantation (alloSCT) by dual-color FISH analysis of X and Y chromosomes. Leuk Res. 2003;27:993–998
  3. Gardiner N, Lawler M, O'Riordan J, De'Arce M, McCann SR. Donor chimaerism is a strong indicator of disease free survival following bone marrow transplantation for chronic myeloid leukaemia. Leukemia. 1997;11(Suppl 3):512–515
  4. Bader P, Beck J, Frey A, et al. Serial and quantitative analysis of mixed hematopoietic chimerism by PCR in patients with acute leukemias allows the prediction of relapse after allogeneic BMT. Bone Marrow Transplant. 1998;21:487–495
  5. Serrano J, Roman J, Sanchez J, et al. Molecular analysis of lineage-specific chimerism and minimal residual disease by RT-PCR of p210(BCR-ABL) and p190(BCR-ABL) after allogeneic bone marrow transplantation for chronic myeloid leukemia: increasing mixed myeloid chimerism and p190(BCR-ABL) detection precede cytogenetic relapse. Blood. 2000;95:2659–2665
  6. Bader P, Kreyenberg H, Hoelle W, et al. Increasing mixed chimerism defines a high-risk group of childhood acute myelogenous leukemia patients after allogeneic stem cell transplantation where pre-emptive immunotherapy may be effective. Bone Marrow Transplant. 2004;33:815–821
  7. Bader P, Niethammer D, Willasch A, Kreyenberg H, Klingebiel T. How and when should we monitor chimerism after allogeneic stem cell transplantation?. Bone Marrow Transplant. 2005;35:107–119
  8. Beck O, Seidl C, Lehrnbecher T, et al. Quantification of chimerism within peripheral blood, bone marrow and purified leukocyte subsets: comparison of singleplex and multiplex PCR amplification of short tandem repeat (STR) loci. Eur J Haematol. 2006;76:237–244
  9. Miura Y, Tanaka J, Toubai T, et al. Analysis of donor-type chimerism in lineage-specific cell populations after allogeneic myeloablative and non-myeloablative stem cell transplantation. Bone Marrow Transplant. 2006;37:837–843
  10. De Rosa SC, Brenchley JM, Roederer M. Beyond six colors: a new era in flow cytometry. Nat Med. 2003;9:112–117
  11. Chattopadhyay PK, Price DA, Harper TF, et al. Quantum dot semiconductor nanocrystals for immunophenotyping by polychromatic flow cytometry. Nat Med. 2006;12:972–977
  12. Petrovas C, Casazza JP, Brenchley JM, et al. PD-1 is a regulator of virus-specific CD8+ T cell survival in HIV infection. J Exp Med. 2006;203:2281–2292
  13. Precopio ML, Betts MR, Parrino J, et al. Immunization with vaccinia virus induces polyfunctional and phenotypically distinctive CD8(+) T cell responses. J Exp Med. 2007;204:1405–1416
  14. Laughlin MJ, Eapen M, Rubinstein P, et al. Outcomes after transplantation of cord blood or bone marrow from unrelated donors in adults with leukemia. N Engl J Med. 2004;351:2265–2275
  15. Rocha V, Labopin M, Sanz G, et al. Transplants of umbilical-cord blood or bone marrow from unrelated donors in adults with acute leukemia. N Engl J Med. 2004;351:2276–2285
  16. Takahashi S, Ooi J, Tomonari A, et al. Comparative single-institute analysis of cord blood transplantation from unrelated donors with bone marrow or peripheral blood stem-cell transplants from related donors in adult patients with hematologic malignancies after myeloablative conditioning regimen. Blood. 2007;109:1322–1330
  17. Terasaki PI, McClelland JD. Microdroplet assay of human serum cytotoxins. Nature. 1964;204:998–1000
  18. Sijpkens YW, Doxiadis , De Fijter JW, et al. Sharing cross-reactive groups of MHC class I improves long-term graft survival. Kidney Int. 1999;56:1920–1927
  19. Pei R, Chen T, Orpilla J, Lee JH. A simultaneous negative and positive selection method that can detect chimerism at a frequency of 1 per 10,000 by flow cytometry. Tissue Antigens. 1997;50:197–201
  20. Metes D, Logar A, Rudert WA, et al. Four-color flow cytometric analysis of peripheral blood donor cell chimerism. Hum Immunol. 2003;64:787–795
  21. Gratama JW, Orfao A, Barnett D, et al. Flow cytometric enumeration of CD34+ hematopoietic stem and progenitor cells. European Working Group on Clinical Cell Analysis. Cytometry. 1998;34:128–142
  22. Lacombe F, Durrieu F, Briais A, et al. Flow cytometry CD45 gating for immunophenotyping of acute myeloid leukemia. Leukemia. 1997;11:1878–1886
  23. Kern W, Voskova D, Schnittger S, Schoch C, Hiddemann W, Haferlach T. Four-fold staining including CD45 gating improves the sensitivity of multiparameter flow cytometric assessment of minimal residual disease in patients with acute myeloid leukemia. Hematol J. 2004;5:410–418
  24. Dobo I, Pineau D, Robillard N, et al. Standardization of the CFU-GM assay: advantages of plating a fixed number of CD34+ cells in collagen gels. J Hematother Stem Cell Res. 2003;12:543–551
  25. Mageed AS, Pietryga DW, DeHeer DH, West RA. Isolation of large numbers of mesenchymal stem cells from the washings of bone marrow collection bags: characterization of fresh mesenchymal stem cells. Transplantation. 2007;83:1019–1026
  26. Schumm M, Feuchtinger T, Pfeiffer M, et al. Flow cytometry with anti HLA-antibodies: a simple but highly sensitive method for monitoring chimerism and minimal residual disease after HLA-mismatched stem cell transplantation. Bone Marrow Transplant. 2007;39:767–773

PII: S1083-8791(08)00145-6

doi: 10.1016/j.bbmt.2008.04.001

Biology of Blood and Marrow Transplantation
Volume 14, Issue 6 , Pages 693-701 , June 2008