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Volume 14, Issue 7 , Pages 766-774 , July 2008

Evaluation of Angiopoietins and Cell-Derived Microparticles after Stem Cell Transplantation

Shosaku Nomura
- Division of Hematology, Kishiwada City Hospital, Osaka, Japan
- Correspondence and reprint requests: Shosaku Nomura, MD, Division of Hematology, Kishiwada City Hospital, 1001, Gakuhara-cho, Kishiwada, Osaka 596-8501, Japan.
Kazuyoshi Ishii
- Division of Hematology, Kishiwada City Hospital, Osaka, Japan
Norihito Inami
- Second Department of Internal Medicine, Kansai Medical University, Moriguchi, Osaka, Japan
Yutaka Kimura
- Second Department of Internal Medicine, Kansai Medical University, Moriguchi, Osaka, Japan
Nobuhiko Uoshima
- Department of Internal Medicine, Matsushita Memorial Hospital, Moriguchi, Osaka, Japan
Hiroyuki Ishida
- Department of Pediatrics, Matsushita Memorial Hospital, Moriguchi, Osaka, Japan
Takao Yoshihara
- Department of Pediatrics, Matsushita Memorial Hospital, Moriguchi, Osaka, Japan
Fumiaki Urase
- Department of Hematology, Kinki University, Sayama, Osaka, Japan
Yasuhiro Maeda
- Department of Hematology, Kinki University, Sayama, Osaka, Japan
Kunio Hayashi
- Division of Hematology, Keihanna Hospital, Hirakata, Osaka, Japan

Received 3 February 2008 ,Accepted 10 April 2008.

Changes of sFasL, EDMP, Ang-1, and Ang-2 in patients undergoing allogeneic and autologous SCT. Blood samples were obtained on days 0 (basal), 7 (1 weeks), 14 (2 weeks), 21 (3 weeks), and 28 (4 weeks)

Changes of sFasL, EDMP, Ang-1, and Ang-2 in patients undergoing allogeneic and autologous SCT. Blood samples were obtained on days 0 (basal), 7 (1 weeks), 14 (2 weeks), 21 (3 weeks), and 28 (4 weeks) after the transplantation. Error bars show standard error. Student's t-test was used for statistical comparisons.
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sFasL and EDMP levels in patients after allogeneic SCT with and without high-Ang-2. Values presented as mean ± standard error.

sFasL and EDMP levels in patients after allogeneic SCT with and without high-Ang-2. Values presented as mean ± standard error.
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Probability of DFS in aGVHD. The estimated probability of DFS at 2 years was 78% (grade I) and 37% (grade II-IV).

Probability of DFS in aGVHD. The estimated probability of DFS at 2 years was 78% (grade I) and 37% (grade II-IV).
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Ang-2 levels in patients after allogeneic SCT with and without high-grade GVHD. Values presented as mean ± standard error.

Ang-2 levels in patients after allogeneic SCT with and without high-grade GVHD. Values presented as mean ± standard error.
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Probability of DFS in relation to Ang-2 levels. The estimated probability of DFS at 2 years was 56% (low-Ang-2) and 44% (high-Ang-2).

Probability of DFS in relation to Ang-2 levels. The estimated probability of DFS at 2 years was 56% (low-Ang-2) and 44% (high-Ang-2).
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Flow cytometric analysis of EDMP. Only particles positive for FITC-labeled Annexin V and PE-CD51 were gated to distinguished endothelial cells and EDMP from electric noise (A, B). EDMPs were elevated

Flow cytometric analysis of EDMP. Only particles positive for FITC-labeled Annexin V and PE-CD51 were gated to distinguished endothelial cells and EDMP from electric noise (A, B). EDMPs were elevated by stimulation of TNF-α (C), and this elevation was inhibited the addition of Ang-1 (D). Furthermore, the suppressive effect of Ang-1 was partially inhibited by the addition of Ang-2 (E).
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