Cellular Immune Reconstitution and Its Impact on Clinical Outcome in Children with β Thalassemia Major Undergoing a Matched Related Myeloablative Allogeneic Bone Marrow Transplant

Reconstitution kinetics of NK, B, T, and dendritic cell subsets in the first year post-SCT. Each line indicates corresponding cellular subsets at appropriate time points post-SCT. Error bars indicate the 25th to 75th percentiles values. Median pretransplant values are shown as a horizontal bar and the baseline error bars service as the reference range (highlighted area). MC, monocytoid dendritic cells; PC, plasmacytoid dendritic cells.

Overview of reconstitution kinetics of lymphocyte subsets and immune cells in the first year post-SCT. Each bar represents total WBC count, whereas lines indicate the corresponding cellular subsets at appropriate time points post-SCT (N = 63). (A) Illustrates reconstitution of total T cells (CD3), helper T cells (CD4), cytotoxic T cells (CD8), and natural killer (NK, CD3⁻CD56⁺CD16⁺). Post-HSCT recovery pattern of B cells (CD19), helper naïve T (CD4CD45RA), helper memory T (CD4CD45RO), cytotoxic naïve T (CD8CD45RA), and cytotoxic memory T (CD8CD45RO) cells are shown in (B), whereas cytokine-induced killer (CIK, CD3⁺CD56⁺CD16⁺) and dendritic cell recovery for both monocytoid (MC, Lin⁻HLADR⁻CD11C⁺) and plasmacytoid cells (PC, Lin⁻HLADR⁻CD123⁺) are depicted in (C).

Median day 28 NK cell counts and secondary graft rejection.

Kaplan-Meier estimates of graft rejection, EFS, and OS by day 28 NK cell groups. (A) Secondary graft rejection. (B) EFS.