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Volume 17, Issue 1 , Pages 69-77 , January 2011

Exogenous Addition of Minor H Antigen HA-1+ Dendritic Cells to Skin Tissues Ex Vivo Causes Infiltration and Activation of HA-1-Specific Cytotoxic T Cells

Yeung-Hyen Kim
- Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
Florry A. Vyth-Dreese
- Division of Immunology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
Ellen Schrama
- Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
Stan Pavel
- Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands
Ingeborg Bajema
- Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands
Els Goulmy
- Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
Eric Spierings
- Department of Immunohematology and Blood Transfusion, Leiden University Medical Center, Leiden, The Netherlands
- Department of Immunology, University Medical Center Utrecht, Utrecht, The Netherlands
- Correspondence and reprint requests: Eric Spierings, PhD, Department of Immunology, University Medical Center Utrecht, P.O. Box 85500, 3508 GA Utrecht, The Netherlands.

Received 11 December 2009 ,Accepted 5 August 2010.

Title	About	File Size
Supplementary Figure S1	Influx of mature and immature DCs in skin explant tissues. CFDA-SE-labeled mature and immature DCs were incubated with skin explant tissues as indicated. The arrows indicate infiltrated CFDA-SE-labeled DCs.	4 MB
Supplementary Figure S2	CD1a-positive cells in explant tissues. (A) Directly snap-frozen skin sample (green = CD8, red = CD1a). (B) Skin incubated O/N with medium, washed, and incubated for 72 hours. (C) Skin incubated O/N with allogenic DC, washed, and incubated for 72 hours. White arrows indicate CD1a-positive cells.	0 MB
Supplementary Figure S3	CD1a-positive cells in explant tissues. (A) Control experiment with female (HY−) skin preincubated with HLA-A2+ female (HY−) mature DCs and subsequent incubation with HLA-A2/HY-specific T cells overnight. (B) Control experiment with female (HY−) skin preincubated with HLA-A2+ male (HY+) mature DCs and subsequent incubation with HLA-A2/HY-specific T cells overnight (green signal = CFDA-SE, blue signal = HLA-A2/HY tetramer, red signal = Ki67). Green arrows indicate the infiltrated mature DCs, white arrows mark HLA-A2/HY-specific T cells, and red arrows point at activated T cells.	0 MB